EIF2A antibody (AA 49-53)

Catalog No. ABIN362970

The Rabbit Polyclonal anti-EIF2A antibody has been validated for WB, IHC and IF. It is suitable to detect EIF2A in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for EIF2A antibody (AA 49-53) (ABIN362970)

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EIF2A Antibody

Binding Specificity: AA 49-53

Specificity: The antibody detects endogenous level of total eIF2a protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 49-53 (E-L-S-R-R) derived from Human eIF2a. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-EIF2A antibody (ABIN362970)

Wu, Li, Lin, Wang, Li, Tie, Song, Guo, Yu, Li: "Salvianolic acid B protects human endothelial cells from oxidative stress damage: a possible protective role of glucose-regulated protein 78 induction." in: Cardiovascular research, Vol. 81, Issue 1, pp. 148-58, (2008) (PubMed).

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: eIF2alpha

Background: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Molecular Weight: 38 kDa

NCBI Accession: NP_004085

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly