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SMAD3 antibody (AA 206-210)

The Rabbit Polyclonal anti-SMAD3 antibody has been validated for IHC and IF. It is suitable to detect SMAD3 in samples from Human, Mouse and Rat.
Catalog No. ABIN363048

Quick Overview for SMAD3 antibody (AA 206-210) (ABIN363048)

Target

See all SMAD3 Antibodies
SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity

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Human, Mouse, Rat

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This SMAD3 antibody is un-conjugated

Application

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Immunohistochemistry (IHC), Immunofluorescence (IF)
  • Binding Specificity

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    AA 206-210

    Specificity

    The antibody detects endogenous level of total Smad3 protein.

    Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

    Immunogen

    Peptide sequence around AA 206-210 (N-L-S-P-N) derived from Human Smad3. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

    Isotype

    IgG
  • Application Notes

    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Target

    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

    Alternative Name

    Smad3

    Background

    Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

    Molecular Weight

    52 kDa

    NCBI Accession

    NP_001138

    UniProt

    P84022

    Pathways

    Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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