The Rabbit Polyclonal anti-Niban antibody has been validated for WB and IF. It is suitable to detect Niban in samples from Human. There is 1 publication available.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Immunogen
peptide sequence around AA 912-916 (K-E-G-E-G) derived from Human Niban. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Ji, Ding, Hawke, Xing, Jiang, Mills, Lu: "AKT-dependent phosphorylation of Niban regulates nucleophosmin- and MDM2-mediated p53 stability and cell apoptosis." in: EMBO reports, Vol. 13, Issue 6, pp. 554-60, (2012) (PubMed).
Target
Niban (FAM129A)
(Family with Sequence Similarity 129, Member A (FAM129A))
Alternative Name
Niban
Background
Regulates phosphorylation of a number of proteins involved in translation regulation including EIF2A, EIF4EBP1 and RPS6KB1. May be involved in the endoplasmic reticulum stress response Vill