ARNT antibody (AA 496-789)

Catalog No. ABIN363204

This anti-ARNT antibody is a Mouse Monoclonal antibody detecting ARNT in WB, IHC, IP, IHC (p), ChIP and GS. Suitable for Human, Mouse, Rat, Cow, Sheep, Pig, Monkey and Ferret.

Quick Overview for ARNT antibody (AA 496-789) (ABIN363204)

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Pig, Monkey, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARNT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS)

Clone: H1beta234

Product Details anti-ARNT Antibody

Binding Specificity: AA 496-789

Specificity: Reacts with human HIF 1 beta /ARNT

Cross-Reactivity: Mouse (Murine), Cow (Bovine), Sheep (Ovine), Rat (Rattus)

Cross-Reactivity (Details): Cross reacts with proteins from bovine, sheep, mouse, rat and ferret. Not tested in other species.

Purification: Purified (protein G)

Immunogen: Fusion protein containing amino acids 496-789 of human HIF-1 beta

Isotype: IgG1 kappa

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ChIP1:10-1:500, IHC1:100, IHC-P 1:100, WB 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.4 mg/mL

Buffer: PBS, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Alternative Name: Hypoxia-Inducible Factor 1-beta

Background: HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits which are bHLH proteins of PAS (PER, ARNT, SIM) family. HIF-1 beta forms heterodimer with AHR, AHRR, HIF1 alpha and EPAS1/HIF2 alpha as well as with other bHLH proteins and its dimerization is required for efficient DNA binding. Interact with TACC3 and NOCA7.

Gene ID: 405

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect