BUB1B antibody

Catalog No. ABIN363218

The Mouse Monoclonal anti-BUB1B antibody is suitable to detect BUB1B in samples from Human and Mouse. It has been validated for WB, IF, FACS, IP and ICC.

Quick Overview for BUB1B antibody (ABIN363218)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 8G1

Product Details anti-BUB1B Antibody

Specificity: Reacts with human and mouse hBUBR1 protein

Purification: Purified (protein G)

Immunogen: A portion of hBUBR1 tagged at the N-terminus with pMAL (residues 1-350 of human BUBR1)

Isotype: IgG1 kappa

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB(1:2000 - 1:5000) FC(1 μg per million cells) ICC/IF(1:100) IP(1:10 - 1:500)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.6 mg/mL

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: Mitotic Checkpoint Serine/threonine-Protein Kinase BUB1 beta

Background: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. Defects in BUB1B are the cause of premature chromatid separation trait (PCS). Defects in BUB1B are the cause of mosaic variegated aneuploidy syndrome type 1 (MVA1).

Gene ID: 701

UniProt: O60566