PRDM1 antibody (AA 199-409)

Catalog No. ABIN363225

This anti-PRDM1 antibody is a Mouse Monoclonal antibody detecting PRDM1 in WB, ELISA, IHC, IP, FACS, ICC and IF. Suitable for Human, Mouse and Pig.

Quick Overview for PRDM1 antibody (AA 199-409) (ABIN363225)

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Reactivity: Human, Mouse, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRDM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 3H2-E8

Product Details anti-PRDM1 Antibody

Binding Specificity: AA 199-409

Specificity: Reacts with ~98 kDa BLIMP-1 from mouse, pig and human

Cross-Reactivity (Details): May cross reacts with the beta form (~80 kDa).

Purification: Purified (protein G)

Immunogen: A fragment of the mouse Blimp-1 (residues 199-409)

Isotype: IgG1

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
- ELISA: 1/100 to 1/2000 - ICC/ IF: 1/50 to 1/100 - IHC: 1/200 -FC: 8 μg/mL (for 2 x 10E6 cells/mL) - WB: 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer: , Sodium azide 0.1 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 6 months

Target Details for PRDM1

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Alternative Name: B Lymphocyte-Induced Maturation Protein-1 (BLIMP-1)

Background: Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus.It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages.Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells.Blimp-1 has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

Gene ID: 639

UniProt: O75626

Pathways: Regulation of Muscle Cell Differentiation