MAP1LC3A antibody (AA 50-150)

Catalog No. ABIN363516

The Rabbit Polyclonal anti-MAP1LC3A antibody (ABIN363516) specifically detects MAP1LC3A in WB, IF, ICC, IP and IHC (p). The antibody is reactive with Human, Mouse, Rat and Zebrafish (Danio rerio) samples.

Quick Overview for MAP1LC3A antibody (AA 50-150) (ABIN363516)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAP1LC3A Antibody

Binding Specificity: AA 50-150

Specificity: Reacts with LC3 from human, mouse, rat and Zebrafish

Cross-Reactivity: Cow (Bovine)

Cross-Reactivity (Details): Predicted to react with Xenopus and bovine based on 100 % sequence homology.

Purification: Antigen affinity purified

Immunogen: Synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 50-150)

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ICC/IF1:100-1:300, IHC1:200-1:400, IHC-P1:200-1:400, IP20 μL / 500 μg of lysate, WB2 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Tris-glycine, 150 mM NaCl, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: Light Chain 3 (LC3)

Background: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Gene ID: 84557

Pathways: Autophagy