MAP1LC3A antibody (AA 50-150)

Catalog No. ABIN363516

This anti-MAP1LC3A antibody is a Rabbit Polyclonal antibody detecting MAP1LC3A in WB, IF, IHC (p), ICC and IP. Suitable for Human, Mouse, Rat and Zebrafish (Danio rerio).

Quick Overview for MAP1LC3A antibody (AA 50-150) (ABIN363516)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-MAP1LC3A Antibody

Binding Specificity: AA 50-150

Specificity: Reacts with LC3 from human, mouse, rat and Zebrafish

Cross-Reactivity: Cow (Bovine)

Cross-Reactivity (Details): Predicted to react with Xenopus and bovine based on 100 % sequence homology.

Purification: Antigen affinity purified

Immunogen: Synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 50-150)

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ICC/IF1:100-1:300, IHC1:200-1:400, IHC-P1:200-1:400, IP20 μL / 500 μg of lysate, WB2 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Tris-glycine, 150 mM NaCl, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: Light Chain 3 (LC3)

Background: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Gene ID: 84557

Pathways: Autophagy