OCT4 antibody (AA 100-200)

Catalog No. ABIN363517

This Rabbit Polyclonal antibody specifically detects OCT4 in WB, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Monkey.

Quick Overview for OCT4 antibody (AA 100-200) (ABIN363517)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-OCT4 Antibody

Binding Specificity: AA 100-200

Specificity: Reacts with Oct-4 protein from human, mouse, rat and primate

Cross-Reactivity: Cow (Bovine), Pig (Porcine)

Cross-Reactivity (Details): Predicted to cross react with bovine (93 % ), feline (93 %), porcine (93 %).

Purification: Purified

Immunogen: Synthetic peptide made to an internal portion of the human OCT4 protein sequence (between residues 100-200)

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- WB : 2 μg/mL - FC: 1:50-1:200 -ICC/IF, 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.85 mg/mL

Buffer: Tris-glycine, NaCl 150 mM buffer, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Octamer-Binding Protein 4 (Oct-4)

Background: Stem cells are crucial ingredients in the progressive restriction of cellular developmental capacity during mammalian embryogenesis. Until recently, only embryonic stem cells (ESC)had been shown to exhibit the potential to differentiate into cells of the all three germ layers. However, adult stem cells (ASC) can regain pluripotency (potential to differentiate into various lineages) if transferred from one stem-cell microenvironment into another.The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development.

UniProt: Q01860

Pathways: Stem Cell Maintenance