SIRT6 antibody (Internal Region)

Catalog No. ABIN363527

The Rabbit Polyclonal anti-SIRT6 antibody has been validated for WB. It is suitable to detect SIRT6 in samples from Human.

Quick Overview for SIRT6 antibody (Internal Region) (ABIN363527)

Target: SIRT6 (Sirtuin 6 (SIRT6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SIRT6 Antibody

Binding Specificity: AA 250-350, Internal Region

Specificity: Reacts with human SIRT6

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Not tested in other species. The immunogen used for the production of this antibody shows 78 % homology with the mouse protein and 71 % homology with the rat protein.

Purification: Purified

Immunogen: Synthetic peptide made to an internal region of the human SIRT6 protein (within residues 250-350)

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- WB : 0.5 to 2 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Tris-citrate, phosphate, ( pH 7-8) buffer, Sodium azide 0.1 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for SIRT6

Target: SIRT6 (Sirtuin 6 (SIRT6))

Background: SIRT6 (SIR2-like protein 6) is a member of the yeast silent information regulator 2 (SIR2) family and possesses both strong ADP-ribosyltransferase activity as well as protein deacetylase activity which is dependent on NAD(+). SIRT6 exerts deacetylase activity towards histone H3K9Ac and H3K56Ac, and alters histone H3 acetylation in telomeric chromatin during cell cycle's S-phase. It deacetylates histone H3K9Ac at NFkB target promoters and downregulates a subset of NFkB target genes. Upon DNA damage, SIRT6 promotes DNA end resection via deacetylation of RBBP8. SIRT6 also serves as a corepressor of the transcription factor HIF1 alpha for regulating the expression of multiple glycolytic genes to control glucose homeostasis, and is required for normal IGF1 serum levels. SIRT6 play critical roles in maintaining telomere integrity, fine-tuning aging-associated gene expression programs, preventing genomic instability, and maintaining metabolic homeostasis, thus impacting on several pathways relevant for cancer, metabolism, aging, cellular senescence and apoptosis.