PIK3C3 antibody (AA 700-850)

Catalog No. ABIN363642

This anti-PIK3C3 antibody is a Rabbit Polyclonal antibody detecting PIK3C3 in WB, IF and ICC. Suitable for Human, Mouse, Rat and Pig.

Quick Overview for PIK3C3 antibody (AA 700-850) (ABIN363642)

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3C3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PIK3C3 Antibody

Binding Specificity: AA 700-850

Specificity: Reacts with human VPS34

Cross-Reactivity: Mouse (Murine), Pig (Porcine)

Cross-Reactivity (Details): Cross reacts with proteins from rat, mouse and porcine. The immunogen sequence has 87 % homology with Xenopus protein. Not tested in other species

Purification: Antigen affinity purified

Immunogen: Synthetic peptide made to an internal portion of the human protein (within residues 700-850)

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- WB : 1/1 000 - ICC/IF: 1/100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, glycerol 50 % , BSA 0.1 %, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 6 months

Target Details for PIK3C3

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Alternative Name: VPS34

Background: VPS34, also known as phosphoinositide-3-kinase p100 subunit or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. VPS34 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. It exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. The activity of VPS34 is enhanced by manganese and can be regulated by nutrients, suggesting an important role of VPS34 in the regulation of mTOR protein synthesis. VPS34 also forms a complex with Beclin1 that promotes autophagy and tumor supression.

Pathways: AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagy