FERMT1 antibody (AA 643-660)

Catalog No. ABIN364270

This Rabbit Polyclonal antibody specifically detects FERMT1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Monkey, Hamster, Xenopus laevis and Pig.

Quick Overview for FERMT1 antibody (AA 643-660) (ABIN364270)

Target: FERMT1 (Fermitin Family Member 1 (FERMT1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Monkey, Hamster, Xenopus laevis, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FERMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FERMT1 Antibody

Binding Specificity: AA 643-660

Specificity: Reacts with residues 643-660 (VHEYIGGYIFLSTRSKDQ) of the 77kD human URP1 protein. Sequence is 100 % conserved between almost all species (mouse, rat, canine, bovine, chimpanzee, Xenopus).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Xenopus, Zebrafish (100%) Platypus, Lizard, Stickleback, Pufferfish (94%) Gibbon, Bat, Chicken, Beetle, Tick (89%) Turkey, Nematode (83%).

Purification: Immunoaffinity purified

Immunogen: Residues 643-660 (VHEYIGGYIFLSTRSKDQ) of the 77kD human URP1 protein. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Xenopus, Zebrafish (100%), Platypus, Lizard, Stickleback, Pufferfish (94%), Gibbon, Bat, Chicken, Beetle, Tick (89%), Turkey, Nematode (83%).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FERMT1

Target: FERMT1 (Fermitin Family Member 1 (FERMT1))

Alternative Name: FERMT1 / Kindlin

Background: Name/Gene ID: FERMT1

Synonyms: FERMT1, C20orf42, DTGCU2, Fermitin family homolog 1, Kindlin syndrome protein, KIND1, Kindlin 1, UNC112A, URP1, UNC112 related protein 1, Fermitin family member 1, Kindlerin, Kindlin, Kindlin-1, Unc-112-related protein 1

Gene ID: 55612

UniProt: Q9BQL6