Complement Factor H antibody

Catalog No. ABIN364283

Product Details anti-Complement Factor H Antibody

Target: Complement Factor H (CFH)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement Factor H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Purified anti-human factor H (beta 1H) (139 kD) monoclonal antibody clone OX-24 inhibits the binding of factor H to surface bound C3. This antibody recognizes the human serum complement protein factor H and a 43-49kD truncated form of factor H present at low levels (1-5 μg/mL) in plasma and urine.

Purification: Protein G purified

Immunogen: Human complement factor H.

Type of Immunogen: Purified protein

Clone: OX-24

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-P (20 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

Target Details for Complement Factor H

Target: Complement Factor H (CFH)

Alternative Name: CFH / Complement Factor H (CFH Products)

Background: Name/Gene ID: CFH

Synonyms: CFH, Adrenomedullin binding protein, AHUS1, AMBP1, ARMS1, ARMD4, Beta-1-H-globulin, Factor H, Factor H-like 1, HF, HF2, H factor 1, H factor 2 (complement), HUS, HF1, Beta-1H, CFHL3, Complement factor H, H factor 1 (complement)

Gene ID: 3075

UniProt: P08603

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin