HSPB6 antibody

Catalog No. ABIN364293

This anti-HSPB6 antibody is a Rabbit Polyclonal antibody detecting HSPB6 in WB, IHC, IP and IHC (p). Suitable for Human, Rat, Mouse, Cow and Rabbit.

Quick Overview for HSPB6 antibody (ABIN364293)

Target: HSPB6 (Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6))

Reactivity: Human, Rat, Mouse, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPB6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HSPB6 Antibody

Brand: IHC-plus™

Specificity: Recognizes HSP20, Mr ~20kD. Species cross-reactivity: Human, mouse, rabbit, rat and bovine.

Purification: Immunoaffinity purified

Immunogen: Native HSP20 purified from human skeletal muscle.

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot Analysis: 1 μg/mL detected HSP20 in RIPA lysates from rabbit skeletal muscle and mouse heart cytosol. Immunoprecipitation: Reported to immunoprecipitate HSP20 from rat heart extract and lysates of bovine carotid artery smooth muscle.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M PBS,  pH 7.0, 0.1 % sodium azide, 30 mg/mL BSA, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HSPB6

Target: HSPB6 (Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6))

Alternative Name: HSPB6 / HSP20

Background: Name/Gene ID: HSPB6

Synonyms: HSPB6, Heat shock protein beta-6, Hsp20

Gene ID: 126393

UniProt: O14558