Podoplanin antibody (AA 1-206)

Catalog No. ABIN364350

The Mouse Monoclonal anti-Podoplanin antibody (Clone 5E2) (ABIN364350) specifically detects Podoplanin in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for Podoplanin antibody (AA 1-206) (ABIN364350)

Target: Podoplanin (PDPN)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5E2

Product Details anti-Podoplanin Antibody

Binding Specificity: AA 1-206

Purification: Protein G purified

Immunogen: Recombinant human PDPN (1-206aa) purified from E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: PDPN / Podoplanin

Background: Name/Gene ID: PDPN

Synonyms: PDPN, AGGRUS, HT1alpha-1, Glycoprotein 36, Glycoprotein, 36-KD, gp36, gp38, OTS8, PA2.26, PA2.26 antigen, Podoplanin, T1A, gp40, HT1A-1, HT1alpha-2, T1-alpha, T1A-2

Gene ID: 10630

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport