14-3-3 theta antibody (AA 1-245)

Catalog No. ABIN364361

The Mouse Monoclonal anti-14-3-3 theta antibody has been validated for WB, IHC, ELISA and IHC (p). It is suitable to detect 14-3-3 theta in samples from Human.

Quick Overview for 14-3-3 theta antibody (AA 1-245) (ABIN364361)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AT1A1

Product Details anti-14-3-3 theta Antibody

Binding Specificity: AA 1-245

Brand: IHC-plus™

Purification: Protein G purified

Immunogen: Recombinant human 14-3-3 tau (1-245aa) purified from E. coli. NCBI Accession No.: NP_006817.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: YWHAQ / 14-3-3 Theta

Background: Name/Gene ID: YWHAQ

Synonyms: YWHAQ, 14-3-3 protein T-cell, 14-3-3 protein theta, 14-3-3 protein tau, 14-3-3, 14-3-3 Tau, 1C5, Protein HS1, HS1, Protein tau

Gene ID: 10971

NCBI Accession: NP_006817

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction