GOSR2 antibody

Catalog No. ABIN364380

This anti-GOSR2 antibody is a Mouse Monoclonal antibody detecting GOSR2 in WB, IHC, IP, ICC and IHC (p). Suitable for Human, Rat, Dog, Rabbit, Chicken, Hamster, Monkey and Pig.

Quick Overview for GOSR2 antibody (ABIN364380)

Target: GOSR2 (Golgi SNAP Receptor Complex Member 2 (GOSR2))

Reactivity: Human, Rat, Dog, Rabbit, Chicken, Hamster, Monkey, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This GOSR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4HAD6

Product Details anti-GOSR2 Antibody

Brand: IHC-plus™

Specificity: Detects an ~27 kD protein, corresponding to the apparent molecular mass of membrin, on SDSPAGE immunoblots in samples from human, monkey, rat, canine, chicken, hamster, pig, and rabbit origins.

Purification: Protein G purified

Immunogen: Recombinant rat membrin with a His6 tag.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ICC (1:500), IHC, IHC-P (20 μg/mL), IP (2 - 4 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF in 50 % glycerol.

Preservative: PMSF

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GOSR2

Target: GOSR2 (Golgi SNAP Receptor Complex Member 2 (GOSR2))

Alternative Name: GOSR2 / Membrin

Background: Name/Gene ID: GOSR2

Synonyms: GOSR2, 27 kDa Golgi SNARE protein, BOS1, Membrin, GS27, EPM6

Gene ID: 9570

UniProt: O14653

Pathways: ER-Nucleus Signaling