Sec8 antibody

Catalog No. ABIN364381

This Mouse Monoclonal antibody specifically detects Sec8 in WB, IHC, IP and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Pig, Dog, Chicken, Monkey, Hamster, Guinea Pig, Sheep and Trout.

Quick Overview for Sec8 antibody (ABIN364381)

Target: Sec8 (EXOC4) (Exocyst Complex Component 4 (EXOC4))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Pig, Dog, Chicken, Monkey, Hamster, Guinea Pig, Sheep, Trout

Host: Mouse

Clonality: Monoclonal

Conjugate: This Sec8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 14G1

Product Details anti-Sec8 Antibody

Specificity: Detects an ~110 kD protein, corresponding to the apparent molecular mass of rSec8 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, chicken, fish (rainbow trout) guinea pig, hamster, monkey, pig, rabbit and sheep origins.

Purification: Protein G purified

Immunogen: Recombinant rat rSec8 (his-tagged) expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP (12.5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF, 50 % glycerol.

Preservative: PMSF

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Sec8

Target: Sec8 (EXOC4) (Exocyst Complex Component 4 (EXOC4))

Alternative Name: EXOC4 / SEC8

Background: Name/Gene ID: EXOC4

Synonyms: EXOC4, Exocyst complex component Sec8, Exocyst complex component 4, KIAA1699, Sec8p, SEC8-like 1 (S. cerevisiae), SEC8, SEC8-like 1, SEC8L1

Gene ID: 60412

UniProt: Q96A65

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis