Syntaxin 12 antibody (Cytoplasmic Domain)

Catalog No. ABIN364383

This anti-Syntaxin 12 antibody is a Mouse Monoclonal antibody detecting Syntaxin 12 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog and Hamster.

Quick Overview for Syntaxin 12 antibody (Cytoplasmic Domain) (ABIN364383)

Target: Syntaxin 12 (STX12)

Reactivity: Human, Mouse, Rat, Dog, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Syntaxin 12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 15G2

Product Details anti-Syntaxin 12 Antibody

Binding Specificity: Cytoplasmic Domain

Brand: IHC-plus™

Specificity: Identifies recombinant rat syntaxin 13 and will detect an ~38 kD protein, corresponding to the apparent molecular mass of syntaxin 13 on SDS-PAGE immunoblots, in samples from mouse, rat, dog and hamster origins. Additional bands of 27 and 37 kD may also be recognized in these lysates, and are believed to correspond to degradation products of syntaxin 13.

Purification: Protein G purified

Immunogen: Recombinant cytoplasmic domain of rat syntaxin 13.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (20 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide in 50 % glycerol. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Syntaxin 12

Target: Syntaxin 12 (STX12)

Alternative Name: Stx13 / Syntaxin 13

Background: Name/Gene ID: STX12

Synonyms: STX12, STX14, Syntaxin 12, Syntaxin-12, STX13, Syntaxin 13

Gene ID: 23673

UniProt: Q86Y82

Pathways: Synaptic Vesicle Exocytosis