STX2 antibody (AA 1-19)

Catalog No. ABIN364385

The Rabbit Polyclonal anti-STX2 antibody has been validated for WB, IHC, IP and IHC (p). It is suitable to detect STX2 in samples from Human, Mouse, Rat, Rabbit, Cow, Pig, Monkey, Hamster, Insect, Chicken, Drosophila melanogaster, Sheep and Xenopus laevis.

Quick Overview for STX2 antibody (AA 1-19) (ABIN364385)

Target: STX2 (Syntaxin 2 (STX2))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Pig, Monkey, Hamster, Insect, Chicken, Drosophila melanogaster, Sheep, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STX2 Antibody

Binding Specificity: AA 1-19

Brand: IHC-plus™

Specificity: Detects an ~35 kD protein, corresponding to the apparent molecular mass of syntaxin 2 on SDSPAGE immunoblots, in samples from human, mouse, rat, bovine, canine, chicken, Drosophila, hamster, insect, monkey, pig, rabbit, sheep and Xenopus origins. The antibody specificity has been confirmed by specific peptide inhibition in immunoblotting studies.

Purification: Immunoaffinity purified

Immunogen: Rat syntaxin 2 synthetic peptide, corresponding to amino acid residues 1-19, conjugated to KLH.

Type of Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), IP (10 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for STX2

Target: STX2 (Syntaxin 2 (STX2))

Alternative Name: STX2 / Syntaxin 2

Background: Name/Gene ID: STX2
Family: Syntaxin/epimorphin

Synonyms: STX2, Epimorphin, EPM, EPIM, STX2B, Syntaxin-2, Stx2A, STX2C, Syntaxin 2

Gene ID: 2054

UniProt: P32856