STEAP1 antibody (AA 1-70)

Catalog No. ABIN364397

The Mouse Monoclonal anti-STEAP1 antibody is suitable to detect STEAP1 in samples from Human. It has been validated for WB, ELISA, IHC, IF, IHC (p) and ICC.

Quick Overview for STEAP1 antibody (AA 1-70) (ABIN364397)

Target: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This STEAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: J2D2

Product Details anti-STEAP1 Antibody

Binding Specificity: AA 1-70

Purification: Protein G purified

Immunogen: Recombinant human STEAP1 (1-70aa) purified from E. coli. NCBI Accession No: NP_036581.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, ICC, IF, IHC, IHC-P (10 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for STEAP1

Target: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Alternative Name: STEAP1 / STEAP

Background: Name/Gene ID: STEAP1

Synonyms: STEAP1, Metalloreductase STEAP1, PRSS24, STEAP

Gene ID: 26872

NCBI Accession: NP_036581

UniProt: Q9UHE8

Pathways: Transition Metal Ion Homeostasis