SIM1 antibody

Catalog No. ABIN364478

The Rabbit Polyclonal anti-SIM1 antibody has been validated for IHC, IHC (p) and ICC. It is suitable to detect SIM1 in samples from Human, Cow and Monkey.

Quick Overview for SIM1 antibody (ABIN364478)

Target: SIM1 (Single-Minded Homolog 1 (SIM1))

Reactivity: Human, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIM1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-SIM1 Antibody

Brand: IHC-plus™

Specificity: Recognizes Sim1. Species cross-reactivity: Human and rodent. MW: 85508.7D.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Bovine (100%) Orangutan, Rabbit, Pig (92%) Elephant, Panda, Bat, Dog, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide: (13aa) 423-435: ADRPGSQHDASCA. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Bovine (100%), Orangutan, Rabbit, Pig (92%), Elephant, Panda, Bat, Dog, Horse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SIM1

Target: SIM1 (Single-Minded Homolog 1 (SIM1))

Alternative Name: SIM1

Background: Name/Gene ID: SIM1

Synonyms: SIM1, BHLHe14, Single-minded homolog 1

Gene ID: 6492

UniProt: P81133