SERPINH1 antibody

Catalog No. ABIN364502

The Mouse Monoclonal anti-SERPINH1 antibody has been validated for WB, ELISA, IHC and IHC (p). It is suitable to detect SERPINH1 in samples from Human, Mouse, Rat, Dog, Pig, Chicken, Cow, Guinea Pig, Rabbit, Sheep, Hamster and Monkey.

Quick Overview for SERPINH1 antibody (ABIN364502)

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Reactivity: Human, Mouse, Rat, Dog, Pig, Chicken, Cow, Guinea Pig, Rabbit, Sheep, Hamster, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This SERPINH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: M16-10A1

Product Details anti-SERPINH1 Antibody

Specificity: Recognizes rat HSP47 at ~47kD. Species cross-reactivity: human, monkey, mouse, rabbit, hamster, guinea pig, bovine, canine, sheep, porcine and chicken (weak).

Purification: Protein G purified

Immunogen: Native rat Hsp47 (Colligin) protein (full length)

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: HeLa Heat Shocked Cell Lysate.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in PBS, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SERPINH1

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Alternative Name: SERPINH1 / HSP47

Background: Name/Gene ID: SERPINH1
Subfamily: Serpin

Synonyms: SERPINH1, 47 kDa heat shock protein, AsTP3, Colligin, Colligin-1, Collagen-binding protein, Colligin-2, CBP1, HSP47, OI10, Serpin H1, SERPINH2, CBP2, Collagen-binding protein 1, Colligen, gp46, PIG14, PPROM, RA-A47

Gene ID: 871

UniProt: P50454