CD19 antibody (FITC)

Catalog No. ABIN370859

The Mouse Monoclonal anti-CD19 antibody has been validated for FACS. It is suitable to detect CD19 in samples from Mouse.

Quick Overview for CD19 antibody (FITC) (ABIN370859)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD19 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MB19-1

Product Details anti-CD19 Antibody (FITC)

Specificity: This antibody is specific to CD19 B cell differentiation antigen (Mr. 95 kDa).

Characteristics: Synonyms: Leu-12, B-cell marker, Differentiation antigen CD19, B-lymphocyte surface antigen B4

Immunogen: CD19+ mouse pre-B cell line, 300. 19. (Ref. 1)

Isotype: IgA

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.1-3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background: CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e., B220+/CD43+/HSA+) through fully differentiated B cell stages. (Ref.1-7) Terminally differentiated plasma cells do not express CD19.1 In humans, the CD19 Molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. (Ref.3,4) All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. (Ref.1,7) Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. (Ref.7)Synonyms: B-cell marker, B-lymphocyte surface antigen B4, Differentiation antigen CD19, Leu-12

Gene ID: 12478

UniProt: P25918

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway