CD28 antibody

Catalog No. ABIN370909

This Hamster Monoclonal antibody specifically detects CD28 in FACS, IP and Func. It exhibits reactivity toward Mouse.

Quick Overview for CD28 antibody (ABIN370909)

Target: CD28

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Clone: PV-1

Product Details anti-CD28 Antibody

Characteristics: Synonyms: TP44, T-cell-specific surface glycoprotein CD28

Purification: Purified

Isotype: IgG

Application Details

Application Notes: Flow Cytometry. (Ref.8-10)Immunoprecipitation. (Ref.8)Functional Assay: In vitro costimulation of T cells and NK cells. (Ref.8,9)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. (Ref.1-4) CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. (Ref.5) CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses. (Ref.6-9)Synonyms: T-cell-specific surface glycoprotein CD28, TP44

UniProt: P31041

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response