IL2 Receptor beta antibody (FITC)

Catalog No. ABIN371155

This Rat Monoclonal antibody specifically detects IL2 Receptor beta in FACS. It exhibits reactivity toward Mouse.

Quick Overview for IL2 Receptor beta antibody (FITC) (ABIN371155)

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL2 Receptor beta antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 5H4

Product Details anti-IL2 Receptor beta Antibody (FITC)

Specificity: This antibody recognises beta chain (Mr. 90-100 kDa) of the IL-2 Receptor. It does not block IL-2 binding. (Ref.1)

Characteristics: Synonyms: Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL2 Receptor beta, P70-75

Immunogen: Rat myeloma YB2/0 transfected with truncated mouse IL-2RB cDNA. (Ref. 1)

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IL2 Receptor beta

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Alternative Name: CD122 / IL2RB

Background: The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the alpha chain which binds IL-2 with low affinity, (ii) the beta chain that binds IL-2 with high affinity, and (ii) the common gamma chain (gc) that does not bind IL-2. (Ref.2) The high affinity receptor complex is an alpha/beta/gamma heterotrimer with a Kd of 1.3 x 10e-11 M. (Ref.2) In mouse spleen, CD122 is expresed on ~30 % of CD8+ cells and all NK cells, but < 1 % of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells. (Ref.4) Cytoplasmic regions of the IL-2R beta chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes. (Ref.1,4)Synonyms: IL-2 receptor subunit beta, IL2 Receptor beta, Interleukin-2 receptor subunit beta, P70-75

Gene ID: 16185

UniProt: P16297

Pathways: JAK-STAT Signaling, Growth Factor Binding