MHC Class I H-2Db antibody

Catalog No. ABIN371175

The Mouse Monoclonal anti-MHC Class I H-2Db antibody (Clone 27-11-13S) (ABIN371175) specifically detects MHC Class I H-2Db in FACS and CyTox. The antibody is reactive with Mouse samples.

Quick Overview for MHC Class I H-2Db antibody (ABIN371175)

Target: MHC Class I H-2Db

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Cytotoxicity Test (CyTox)

Clone: 27-11-13S

Product Details

Specificity: This antibody recognizes the alpha 3 domain of H-2Db class I MHC antigen. It cross-reacts with the alpha 3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed. Customer feedback: Clone 27-11-13S does not cross-react with human cells.

Characteristics: Synonyms: H-2D(B), H2-D1, H-2 class I histocompatibility antigen, D-B alpha chain

Purification: Purified

Immunogen: C3H alphaBDF1 splenocytes. (Ref. 1)

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry. (Ref.1-3)Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections only). Complement-mediated cytotoxicity. (Ref.1,2)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: MHC Class I H-2Db

Alternative Name: MHC Class I H-2 Db

Background: In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant beta 2 Microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.Synonyms: D-B alpha chain, H-2 class I histocompatibility antigen, H-2D(B), H2-D1

Gene ID: 14964

UniProt: P01899