CD3 antibody (Biotin)

Catalog No. ABIN371288

The Mouse Monoclonal anti-CD3 antibody has been validated for FACS. It is suitable to detect CD3 in samples from Pig.

Quick Overview for CD3 antibody (Biotin) (ABIN371288)

Target: CD3

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: BB23-8E6

Product Details anti-CD3 Antibody (Biotin)

Specificity: This antibody recognizes Porcine CD3e (Mr. 20 kDa). The Monoclonal antibody BB238E6 is Mitogenic when presented to peripheral blood mononuclear cells in immobilized form. (Ref.1)

Characteristics: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains (designated as gamma, delta, epsilon, zeta and eta) ranging in size from 16-28 kDa. CD3 epsilon is closely associated with the T cell antigen receptor (TCR), and is present on thymocytes, CD8+ and CD4+ positive cells. The CD3 epsilon complex mediates signal transduction. It plays a major role in signaling during antigen recognition, leading to T cell activation. The TCR/CD3E complex of T lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3 epsilon is expressed on all T cells of all mouse strains.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 397455

UniProt: Q7YRN2

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway