CD8 antibody

Catalog No. ABIN371299

This Mouse Monoclonal antibody specifically detects CD8 in FACS, IP and CyTox. It exhibits reactivity toward Pig.

Quick Overview for CD8 antibody (ABIN371299)

Target: CD8

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox)

Clone: 11-02-76

Product Details anti-CD8 Antibody

Specificity: This antibody reacts with Porcine CD8 alpha (Mr. 32-34 kDa). (Ref-1-3)

Characteristics: Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain

Purification: Purified

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry. (Ref.1-3)Immunoprecipitation Cytotoxicity Assays (Ref.1,2)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. CD8 is expressed on most thymocytes and approximately 1/3 of peripheral blood T cells in humans. CD8 alpha/beta heterodimers are expressed only on TCR alpha/beta T cells, whereas CD8 alpha homodimers can be expressed on alpha/beta and gamma/delta T cells and some NK cells in humans. The pattern of expression in other species has not been well documented.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 396627

UniProt: Q6R2U9