VSIG1 antibody

Catalog No. ABIN371446

This Mouse Monoclonal antibody specifically detects VSIG1 in FACS, Func and IP. It exhibits reactivity toward Chicken.

Quick Overview for VSIG1 antibody (ABIN371446)

Target: VSIG1 (V-Set and Immunoglobulin Domain Containing 1 (VSIG1))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This VSIG1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP)

Clone: CT1

Product Details anti-VSIG1 Antibody

Specificity: This antibody is specific to ChT1 antigen (Mr 63/104 kDa) on Chicken thymocyte. (Ref.1) CT1 can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors cultured on stromal cell monolayers. (Ref.4) CT1 also recognizes quail cortical thymocytes. (Ref.1)

Characteristics: Synonyms: Cell surface A33 antigen, Glycoprotein A34, GPA34, V-set and immunoglobulindomain-containing protein 1

Purification: Purified

Immunogen: Chicken thymocytes.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. Identification and enumeration of ChT1+ cells. (Ref.4)Immunoprecipitation. (Ref.1,4)In vitro Inhibition of T cell differentiation. (Ref.4).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for VSIG1

Target: VSIG1 (V-Set and Immunoglobulin Domain Containing 1 (VSIG1))

Alternative Name: VSIG1

Background: ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90 % of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent thymic emigrants) are ChT1 positive. (Ref.1-4) Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function. (Ref.3)Synonyms: Cell surface A33 antigen, GPA34, Glycoprotein A34, V-set and immunoglobulin domain-containing protein 1

Gene ID: 414795

UniProt: Q9PWR4