TOLLIP antibody

Catalog No. ABIN371662

This Mouse Monoclonal antibody specifically detects TOLLIP in WB, EIA and FACS. It exhibits reactivity toward Human.

Quick Overview for TOLLIP antibody (ABIN371662)

Target: TOLLIP (Toll Interacting Protein (TOLLIP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TOLLIP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Flow Cytometry (FACS)

Clone: SB40a

Product Details anti-TOLLIP Antibody

Specificity: This antibody is specific to Human Tollip. Does not react to TLR2, TLR4, TLR6, TLR7, TLR8 and TLR10 recombinant proteins.

Characteristics: Synonyms: Toll-interacting protein

Purification: Purified

Immunogen: Recombinant Tollip.

Isotype: IgG2a

Application Details

Application Notes: ELISA. Western Blot. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TOLLIP

Target: TOLLIP (Toll Interacting Protein (TOLLIP))

Alternative Name: TOLLIP

Background: Toll like protein is a component of the signaling pathway of IL1 and Toll like receptors. It inhibits cell activation by microbial products. Tollip recruits IRAK1 to the IL1 receptor complex and inhibits IRAK1 phosphorylation and kinase activity. It oligomerizes and binds to TLR2 and the TLR4-MD2 complex via its C terminus. It exists as a complex with IRAK1 in unstimulated cells. Upon IL1 signaling, Tollip binds to the activated IL1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP.Synonyms: Toll-interacting protein

Gene ID: 54472

UniProt: Q9H0E2