AIRE antibody (Internal Region)

Catalog No. ABIN372242

The Goat Polyclonal anti-AIRE antibody is suitable to detect AIRE in samples from Human, Rat and Dog. It has been validated for WB, IHC (p) and EIA.

Quick Overview for AIRE antibody (Internal Region) (ABIN372242)

Target: AIRE (Autoimmune Regulator (AIRE))

Reactivity: Human, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-AIRE Antibody

Binding Specificity: Internal Region

Sequence: C-KDVDLSQPRK GRKP

Specificity: This antibody recognize Apeced Protein (AIRE).

Cross-Reactivity (Details): Species reactivity (expected):Dog (Canine) and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to the Internal region of Human AIRE.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/32000. Western Blot: 0.5-1 μg/mL. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris Saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE / APECED

Background: AIRE is a transcriptional regulator that is thought to be important in controlling tissue-specific expression of genes in the thymus. The precise function of the protein is not well defined. It contains zinc finger motifs and isoform 1 is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).Synonyms: Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator

Gene ID: 326

NCBI Accession: NP_000374

UniProt: O43918

Pathways: Chromatin Binding