AQP2 antibody (N-Term)

Catalog No. ABIN372293

The Rabbit Polyclonal anti-AQP2 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect AQP2 in samples from Human.

Quick Overview for AQP2 antibody (N-Term) (ABIN372293)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-AQP2 Antibody

Binding Specificity: N-Term

Specificity: This antibody recognizes Aquaporin 2 (N-term).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to N-terminal residues of Human Aquaporin 2.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 20 μg/mL. Western Blot: 1 μg/mL. ELISA: 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.01 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Alternative Name: Aquaporin-2

Background: Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein aquaporins. Aquaporin 0, and Aquaporin 1 have been the foundation of the growing family of aquaporins. The lens specific Aquaporin 0 represents up to 80 % of total lens membrane protein. Defects in Aquaporin 0 are a cause of autosomal dominant cataract. The lens opacity mutation is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin 0 is a 263 amino acid transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C terminus are predicted to be cytoplasmic. Aquaporin 2 is located in the collecting tubule.Synonyms: ADH water channel, AQP-CD, AQP2, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct

Molecular Weight: 29 kDa.

Gene ID: 359

NCBI Accession: NP_000477

UniProt: P41181

Pathways: Response to Water Deprivation