GCLC antibody (AA 295-313)

Catalog No. ABIN372566

The Rabbit Polyclonal anti-GCLC antibody has been validated for WB, IP, IHC (p) and EIA. It is suitable to detect GCLC in samples from Human, Mouse, Rat, Cow and Monkey.

Quick Overview for GCLC antibody (AA 295-313) (ABIN372566)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivity: Human, Mouse, Rat, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GCLC Antibody

Binding Specificity: AA 295-313

Specificity: Recognizes the heavy subunit of GCS.

Cross-Reactivity (Details): Species reactivity (expected):Human, Monkey, Mouse and Rat, Bovine (weak).
Species reactivity (tested):Rat.

Purification: Protein A Chromatography.

Immunogen: Synthetic peptide derived from corresponding to aa 295-313 of Rat GCLC (heavy subunit of GCS).

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM PBS, pH 7.4 containing 0.09 % Sodium Azide as presrevtaive and 40 % Glycerol, 0.2 % BSA as stabilizers..

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (Add 40-50% glycerol) at-20 °C for longer.

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: GCLC / GLCLC

Background: Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2 mM and increases the inhibitory constant for GSH.Synonyms: GCS heavy chain, GLCL, GLCLC, Gamma-ECS, Gamma-glutamylcysteine synthetase, Glutamate-cysteine ligase catalytic subunit

Gene ID: 2729

NCBI Accession: NP_001184044

UniProt: P48506

Pathways: Cell RedoxHomeostasis