ALOX5 antibody (pSer523)

Catalog No. ABIN372586

This Rabbit Polyclonal antibody specifically detects ALOX5 in WB. It exhibits reactivity toward Human, Rat and Primate.

Quick Overview for ALOX5 antibody (pSer523) (ABIN372586)

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reactivity: Human, Rat, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ALOX5 Antibody

Binding Specificity: pSer523

Specificity: Recognizes the ~80k 5-LO phosphorylated at Ser523 in Western blots.

Cross-Reactivity (Details): Species reactivity (expected):non-Human Primates.
Species reactivity (tested):Human and Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser523 of human 5-Lipoxygenase (5-LO).

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: ALOX5 / LOG5

Background: The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.Synonyms: 5-LO, 5-lipoxygenase, Arachidonate 5-lipoxygenase

Gene ID: 240

NCBI Accession: NP_000689

UniProt: P09917