GFAP antibody

Catalog No. ABIN372651

This anti-GFAP antibody is a Chicken Polyclonal antibody detecting GFAP in WB, IF and IHC (fro). Suitable for Human, Rat and Mouse.

Quick Overview for GFAP antibody (ABIN372651)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GFAP Antibody

Specificity: Specific for the ~50 kDa GFAP protein. A lower band at ~45 kDa is a proteolytic fragment derived from the GFAP molecule.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Ig Fraction

Immunogen: Recombinant and purified Bovine GFAP.

Isotype: IgY

Application Details

Application Notes: Western blot: 1/10,000. Immunofluorescence: 1/1,000. Immunohistochemitsry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2).Synonyms: Glial Fibrillary Acidic Protein

Gene ID: 24387

NCBI Accession: NP_058705

UniProt: P47819