NSF antibody (C-Term)

Catalog No. ABIN372691

This Rabbit Polyclonal antibody specifically detects NSF in WB. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for NSF antibody (C-Term) (ABIN372691)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NSF Antibody

Binding Specificity: C-Term

Specificity: This antibody recognizes the ~75k NSF protein.

Cross-Reactivity (Details): Species reactivity (expected):Human and Mouse.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide corresponding to amino acid residues from the C-terminal region of rat NSF, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: NSF

Background: NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).Synonyms: N-ethylmaleimide-sensitive fusion protein, Vesicle-fusing ATPase, Vesicular-fusion protein NSF

Gene ID: 4905

NCBI Accession: NP_006169

UniProt: P46459