OLIG2 antibody

Catalog No. ABIN372693

This anti-OLIG2 antibody is a Rabbit Polyclonal antibody detecting OLIG2 in WB, IP, IF and IHC (fro). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 4+ publications.

Quick Overview for OLIG2 antibody (ABIN372693)

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OLIG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-OLIG2 Antibody

Specificity: This antibody recognizes the ~32 kDa Olig2 protein in Western blots.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Protein A Chromatography.

Immunogen: Recombinant Mouse Olig2.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/3000. Immunohistochemistry: 1/1000. Immunocytochemistry: 1/1000. Immunoprecipitation: 1/500-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM Glycine, 200 mM Tris, pH 8.0 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-OLIG2 antibody (ABIN372693)

Norwood, Franklin, Sharma, DMello: "Histone deacetylase 3 is necessary for proper brain development." in: The Journal of biological chemistry, Vol. 289, Issue 50, pp. 34569-82, (2014) (PubMed).

Bains, Tourkina, Atkinson, Joseph, Tholanikunnel, Chu, Riemer, Martin, Hoffman: "Loss of caveolin-1 from bronchial epithelial cells and monocytes in human subjects with asthma." in: Allergy, Vol. 67, Issue 12, pp. 1601-4, (2012) (PubMed).

Davies, Goebel-Goody, Coultrap, Browning: "Long term synaptic depression that is associated with GluR1 dephosphorylation but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33138-46, (2008) (PubMed).

Guan, Tkatch, Surmeier, Armstrong, Foehring: "Kv2 subunits underlie slowly inactivating potassium current in rat neocortical pyramidal neurons." in: The Journal of physiology, Vol. 581, Issue Pt 3, pp. 941-60, (2007) (PubMed).

Target Details for OLIG2

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Alternative Name: OLIG2

Background: Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).Synonyms: BHLHB1, Class B basic helix-loop-helix protein 1, OLIG2, Oligo2, Oligodendrocyte transcription factor 2, PRKCBP2, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, RACK17, bHLHB1

Gene ID: 10215

NCBI Accession: NP_005797

UniProt: Q13516