SYT1 antibody (pSer309)

Catalog No. ABIN372725

The Rabbit Polyclonal anti-SYT1 antibody has been validated for WB, IHC (fro) and DB. It is suitable to detect SYT1 in samples from Human, Rat, Mouse, Cow, Chicken, Dog, Zebrafish (Danio rerio) and Non-Human Primate.

Quick Overview for SYT1 antibody (pSer309) (ABIN372725)

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Human, Rat, Mouse, Cow, Chicken, Dog, Zebrafish (Danio rerio), Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Product Details anti-SYT1 Antibody

Binding Specificity: pSer309

Specificity: This antibody recognizes the ~60k-62k Synaptotagmin protein phosphorylated at Ser309 in Western Blots of Rat brain lysate.

Cross-Reactivity (Details): Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse, non-Human Primates and Zebrafish.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser309 of Synaptotagmin.

Isotype: IgG

Application Details

Application Notes: Dot blot: 1/1,000. Western blot: 1/1,000. Immunohistochemistry: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: Synaptotagmin-1

Background: Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001, Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999, Lee et al., 2004).Synonyms: SYT1, SYT2, SYT3, SYT4, SYT5

Gene ID: 6857

NCBI Accession: NP_001129277

UniProt: P21579

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport