ADAM12 antibody (N-Term)

Catalog No. ABIN372949

This Goat Polyclonal antibody specifically detects ADAM12 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for ADAM12 antibody (N-Term) (ABIN372949)

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ADAM12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ADAM12 Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to A Disintegrin And Metalloproteinase Domain 12 (ADAM12).

Purification: Immunoaffinity Chromatography

Immunogen: Peptide sequence AARPLPVSPARALC from the N-Terminal region of ADAM-12

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 3.75 μg/mL. Western blot: 0.1 - 0.3 μg/mL. ELISA: 1/64000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for ADAM12

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Alternative Name: ADAM12 / MLTN

Background: ADAM12 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. Two forms of ADAM12 have been described: ADAM12S and ADAM12L (short and long forms). The short form is a soluble form lacking the transmembrane and cytoplasmic domains. The short form of ADAM12 was reported to provoke myogenesis, and the lack of cytoplasmic domain suggests different regulation pathways (although both forms can be expressed in the same tissue). Other papers investigated the SH3 ligand domains in the cytoplasmic portion of ADAM12, demonstrating regulation routes for ADAM12 via Src and Src tyrosine kinase. ADAM12 is primarily expressed in muscle and bone (although placenta, osteoblasts, and many tumor cell lines also express ADAM12). ADAM12 supports cell adhesion by acting as a cell attachment molecule and binding integrins through the cysteine rich domain. A marker of skeletal muscle regeneration, it has been implicated in myoblast differentiation and fusion. ADAM12 contains the canonical HExxHxxxxxH zinc metalloproteinase motif. Full length ADAM12 (909 amino acids) has a predicted mass of 99.5 kDa, but due to glycosylation and cyteine rich regions, the reduced protein migrates to 114 kDa (unprocessed) and 84 kDa (processed). The soluble form of ADAM12 (738 amino acids, predicted mass of 80.5 kDa) is seen as a 92 kDa zymogen and 68 kDa furin processed form.Synonyms: Disintegrin and metalloproteinase domain-containing protein 12, Meltrin alpha

Gene ID: 8038

NCBI Accession: NP_003465

UniProt: O43184

Pathways: EGFR Signaling Pathway