MELK antibody (C-Term)

Catalog No. ABIN374073

This anti-MELK antibody is a Goat Polyclonal antibody detecting MELK in WB and EIA. Suitable for Human, Mouse, Cow and Dog.

Quick Overview for MELK antibody (C-Term) (ABIN374073)

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reactivity: Human, Mouse, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This MELK antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-MELK Antibody

Binding Specificity: C-Term

Sequence: KRLVEDILSS CKV

Specificity: This antibody detects MELK at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Canine (Dog), Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Syntehtic Peptide from the C Terminus of the protein sequence according to NP_055606.1. Genename: MELK

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3´μg/mL. Experiments gave bands at approx 75 kDa and 150 kDa inhuman testis lysates and in cell lines HeLa and Jurkat after 0.1 μg/mL antibody staining. The 150 kDa band is explained by glycosylation of this member of the AMPK family. Thisprotein has a calculated MW of 74.6 kDa according to NP_055606.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containng 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MELK

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Alternative Name: MELK

Background: MELK, maternal embryonic leucine zipper kinase, belongs to the protein kinase superfamily. It contains 1 protein kinase domain and is expressed in placenta, kidney, thymus, testis, ovary and intestine. MELK phosphorylates ZNF622 and may contribute to its redirection to the nucleus. It may also be involved in the inhibition of spliceosome assembly during mitosis.Synonyms: KIAA0175, Maternal embryonic leucine zipper kinase, hMELK

Gene ID: 9833

NCBI Accession: NP_055606

UniProt: Q14680