SET/TAF-I antibody (N-Term)

Catalog No. ABIN374103

The Goat Polyclonal anti-SET/TAF-I antibody (ABIN374103) specifically detects SET/TAF-I in WB. The antibody is reactive with Human samples.

Quick Overview for SET/TAF-I antibody (N-Term) (ABIN374103)

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SET/TAF-I antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SET/TAF-I Antibody

Binding Specificity: N-Term

Sequence: SAPAAKVSKK ELNS-C

Specificity: This antibody detects SET / I2PP2A at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Bovine (Cow)Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_003002.2

Application Details

Application Notes: Peptide ELISA: Limit Dilution 1/1000. Western blot: 1-3 μg/mL. Approx 20-25 kDa and 45 kDa bands seen in Human Kidney

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SET/TAF-I

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Alternative Name: SET / I2PP2A

Background: SET belongs to a family of multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two named isoforms produced by alternative splicing: Isoform 1 and Isoform 2. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins, however, isoform 2 specific activity is higher. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6,9)(q21,q34.1) with NUP214/CAN.Synonyms: I-2PP2A, IGAAD, Inhibitor of granzyme A-activated DNase, PHAPII, Phosphatase 2A inhibitor I2PP2A, TAF-I, Template-activating factor I

Gene ID: 6418

NCBI Accession: NP_003002

UniProt: H0UI37

Pathways: Apoptosis