ARFGAP3 antibody (C-Term)

Catalog No. ABIN374129

The Goat Polyclonal anti-ARFGAP3 antibody is suitable to detect ARFGAP3 in samples from Human, Mouse, Rat, Dog and Cow. It has been validated for WB.

Quick Overview for ARFGAP3 antibody (C-Term) (ABIN374129)

Target: ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ARFGAP3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARFGAP3 Antibody

Binding Specificity: C-Term

Sequence: C-NGVVTSIQDR YGS

Specificity: This antibody detects ARFGAP3 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_055385.3, NP_001135765.1 Genename: ARFGAP3

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 8000. Western blot: 1-3 μg/mL. Approx 55-60 kDa band seen in mouse brain extracts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for ARFGAP3

Target: ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))

Alternative Name: ARFGAP3

Background: The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which is thought to interact with ADP-ribosylation factor 1 (ARF1). The encoded protein likely promotes hydrolysis of ARF1-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.Synonyms: ADP-ribosylation factor GTPase-activating protein 3, ARF GAP 3, ARFGAP1

Gene ID: 26286

NCBI Accession: NP_055385

UniProt: Q9NP61