DUSP8 antibody (N-Term)

Catalog No. ABIN374169

The Goat Polyclonal anti-DUSP8 antibody (ABIN374169) specifically detects DUSP8 in WB. The antibody is reactive with Human, Mouse, Rat and Cow samples.

Quick Overview for DUSP8 antibody (N-Term) (ABIN374169)

Target: DUSP8 (Dual Specificity Phosphatase 8 (DUSP8))

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This DUSP8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DUSP8 Antibody

Binding Specificity: N-Term

Sequence: *AGDRLPRKVM DAK-C

Specificity: This antibody detetcs Dual specificity protein phosphatase 8 at N-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_004411.2 Genename: DUSP8

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 4000. Western blot: 1-3 μg/mL. Approx 60-65 kDa bands seen in Human Heart lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for DUSP8

Target: DUSP8 (Dual Specificity Phosphatase 8 (DUSP8))

Background: DUSP8 is a ubiquitin specific protease that plays an important regulatory role at the level of protein turnover by preventing degradation. USP8 is involved in cell proliferation, and probably regulates the stability of STAM2 and RASGRF1. USP8 may regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and STAM2. As revealed by structur/function studies, USP8 forms a ternary complex with RNF128 and OTUB1, and interacts with the SH3 domain of STAM2 and RASGRF1. Expression of USP8 is induced Upon growth stimulation in starved human fibroblasts, and expression decreases in response to growth arrest induced by cell-cell contact.Synonyms: Dual specificity protein phosphatase 8

Gene ID: 1850

NCBI Accession: NP_004411

UniProt: Q13202