KLF3 antibody (N-Term)

Catalog No. ABIN374196

The Goat Polyclonal anti-KLF3 antibody (ABIN374196) specifically detects KLF3 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog and Pig samples.

Quick Overview for KLF3 antibody (N-Term) (ABIN374196)

Target: KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This KLF3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KLF3 Antibody

Binding Specificity: N-Term

Sequence: LMFDPVPVKQ EAMD-C

Specificity: This antibody detects KLF3 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Porcine, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_057615.3 Genename: KLF3

Application Details

Application Notes: Peptide ELISA: 1/32000. Western blot: 1 - 3 μg/mL. Approx 40 kDa band observed in Human Heart lysates (predictedMW of 40 kDa according to NP_057615). Please note that the 40 kDa Band is sometimesaccompanied by a 80-90 kDa band of unknown identity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KLF3

Target: KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))

Alternative Name: KLF3

Background: The human homolog is a transcription factor involved in haematopoietic differentiation. It binds to the CACCC box.Synonyms: BKLF, Basic krueppel-like factor, CACCC-box-binding protein BKLF, KLF3, Krueppel-like factor 3, TEF-2

Gene ID: 51274

NCBI Accession: NP_057615

UniProt: P57682