Iba1 antibody (C-Term, Isoform 1)

Catalog No. ABIN374206

This Goat Polyclonal antibody specifically detects Iba1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Rat, Mouse and Pig.

Quick Overview for Iba1 antibody (C-Term, Isoform 1) (ABIN374206)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human, Rat, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Iba1 Antibody

Binding Specificity: C-Term, Isoform 1

Specificity: This antibody is expected to recognizes Isoform 1 (NP_116573.1) and Isoform 3 (NP_001614.3) of AIF1.

Cross-Reactivity (Details): Species reactivity (expected):Human, Porcine.
Species reactivity (tested):Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3. Genename: AIF1

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx 16 kDa band observed in Rat Brain lysates (calculatedMW of 16.6 kDa according to Human NP_001614.3 and of 16.6 kDa according to RatNP_058892.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

Gene ID: 199

NCBI Accession: NP_116573

UniProt: P55008

Pathways: Smooth Muscle Cell Migration