HLTF antibody (C-Term)

Catalog No. ABIN374218

This Goat Polyclonal antibody specifically detects HLTF in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog and Cow.

Quick Overview for HLTF antibody (C-Term) (ABIN374218)

Target: HLTF (Helicase-Like Transcription Factor (HLTF))

Reactivity: Human, Rat, Mouse, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This HLTF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HLTF Antibody

Binding Specificity: C-Term

Sequence: C-KQAKINEIRT LIDL

Specificity: This antibody detects RNF80 / HLTF at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dag, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_003062.2, NP_620636.1 Genename: HLTF

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.2-1 μg/mL. Approx 120 kDa and 50 kDa bands seen in Jurkat cell lysate. Please note that the weaker smaller band may represent a novel isoform of this proteinand is in agreement with previous Western blot observations (J Biol Chem 1995 Mar3,270(9): 4575-87).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for HLTF

Target: HLTF (Helicase-Like Transcription Factor (HLTF))

Alternative Name: SMARCA3 / HLTF

Background: Synonyms: DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, HIP116A, Helicase-like transcription factor, RING finger protein 80, RNF80, SNF2L3, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, Sucrose nonfermenting protein 2-like 3, ZBU1

Gene ID: 6596

NCBI Accession: NP_003062

UniProt: Q14527