Melanophilin antibody (C-Term)

Catalog No. ABIN374219

The Goat Polyclonal anti-Melanophilin antibody is suitable to detect Melanophilin in samples from Mouse and Rat. It has been validated for WB, IF and EIA.

Quick Overview for Melanophilin antibody (C-Term) (ABIN374219)

Target: Melanophilin (MLPH)

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Melanophilin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-Melanophilin Antibody

Binding Specificity: C-Term

Sequence: C-ARHIFAKPVM AQQP

Specificity: This antibody detects Melanophilin at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_443748.2. Genename: Mlph

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 80 kDa band observed in Mouse Melanocyte extracts. Please note that this product may require overnight incubation of primary and high proteinloads. Immunofluorescence: Successfully used at 0.5 μg/mL to stain mouse melanocytes, seepicture. See also publication Figueiredo AC et al. , J Biol Chem.2008 Aug22,283(34): 23209-16. Epub 2008 Jun 17. (PMID: 18559336). GST-pull down assay: see publication Figueiredo AC et al. , J Biol Chem.2008 Aug22,283(34): 23209-16. Epub 2008 Jun 17. (PMID: 18559336).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Melanophilin

Target: Melanophilin (MLPH)

Alternative Name: Melanophilin

Background: Melanophilin is a member of the exophilin subfamily of Rab effector proteins. It forms a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.Synonyms: Exophilin-3, Leaden protein, Ln, Mlph, Slac2a, Slp homolog lacking C2 domains a, Synaptotagmin-like protein 2a

Gene ID: 171531

NCBI Accession: NP_443748

UniProt: Q91V27