GULP1 antibody (C-Term)

Catalog No. ABIN374220

The Goat Polyclonal anti-GULP1 antibody (ABIN374220) specifically detects GULP1 in WB and IHC (p). The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for GULP1 antibody (C-Term) (ABIN374220)

Target: GULP1 (GULP, Engulfment Adaptor PTB Domain Containing 1 (GULP1))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GULP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GULP1 Antibody

Binding Specificity: C-Term

Sequence: EGTVFCLDPL DSRC

Specificity: This antibody detetcs GULP / GULP1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, DogSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_057399.1. Genename: GULP1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.2-0.6 μg/mL. Approx 35 kDa band observed in Human Placenta lysates(calculated MW of 34.5 kDa according to NP_057399.1). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Kidney shows staining ofcytoplasm, stronger in a selection of epithelial cells of distal convoluted tubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for GULP1

Target: GULP1 (GULP, Engulfment Adaptor PTB Domain Containing 1 (GULP1))

Alternative Name: GULP1 / CED6

Background: The prompt clearance of cells undergoing apoptosis is critical during embryonic development, normal tissue turnover, as well as inflammation and autoimmunity. CED6 and its human homologue gulp, encode an adapter protein, whose function in engulfment. The upstream and downstream components of CED6 mediated signaling are not known. Recently, CED1 has been shown to encode a transmembrane protein on phagocytic cells, with two functional sequence motifs in its cytoplasmic tail that are important for engulfment.Synonyms: CED-6, Cell death protein 6 homolog, PTB domain adapter protein CED-6, PTB domain-containing engulfment adapter protein 1

Gene ID: 51454

NCBI Accession: NP_057399

UniProt: Q9UBP9