SF3B3 antibody (C-Term)

Catalog No. ABIN374246

The Goat Polyclonal anti-SF3B3 antibody is suitable to detect SF3B3 in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB.

Quick Overview for SF3B3 antibody (C-Term) (ABIN374246)

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SF3B3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SF3B3 Antibody

Binding Specificity: C-Term

Sequence: C-VSKKLEDIRT RYAF

Specificity: This antibody detects SAP130 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_036558.3 Genename: SF3B3

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 2000. Western blot: 0.1-0.3 μg/mL. Approx 150 kDa band observed in nuclear lysates of cell lineHeLa (calculated MW of 130 kDa according to NP_036558.3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for SF3B3

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Alternative Name: SF3B3 / SAP130

Background: Synonyms: KIAA0017, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SAP 130, SAP-130, SF3b130, STAF130, Spliceosome-associated protein 130, Splicing factor 3B subunit 3

Gene ID: 23450

NCBI Accession: NP_036558

UniProt: Q15393