SART1 antibody (N-Term)

Catalog No. ABIN374288

The Goat Polyclonal anti-SART1 antibody is suitable to detect SART1 in samples from Human, Mouse, Rat and Cow. It has been validated for WB.

Quick Overview for SART1 antibody (N-Term) (ABIN374288)

Target: SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This SART1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SART1 Antibody

Binding Specificity: N-Term

Sequence: GSSKKHRGEK EAA-C

Specificity: This antibody detects SART1 at N-term. It is expected to recognise both the human SART1(800) and SART1(259) proteins.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_005137.1 Genename: SART1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.03-0.1 μg/mL. Approx 125 kDa band observed in HeLa lysates (consistentwith Vertegaal et al, below, calculated MW of 90.3 kDa according to NP_005137).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for SART1

Target: SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))

Alternative Name: SART1

Background: Synonyms: Hom s 1, Homs-1, SART-1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein, U4/U6.U5 tri-snRNP-associated protein 1

Gene ID: 9092

NCBI Accession: NP_005137

UniProt: O43290

Pathways: Ribonucleoprotein Complex Subunit Organization